|
TPN Solutions which consists of dextrose, amino acids, electrolytes, vitamins and trace elements are provided for the hospitalized patients with the purpose of nutritional support. To assure safety, it is advised that TPN solution should be mixed in a laminar air flow unit by aseptic techniques. The stability of water soluble vitamins in TPN solution are questionable. According to recent studies, water soluble vitamins are not stable in the TPN solution with bisulfite which is used as preservatives. Therefore it is necessary to assess the stability of vitamins in TPN solution. Two kinds of SNUH standard formulas, one is IVH-1 with the presence of 0.008％ bisulfite and the other is IVH-3 with the presence of 0.05％ bisulfite, were stored in 1000㎖ plastic containers under condition of refrigeration and protection from light. And then the amount of remaining water soluble vitamins in TPN solutions were measured by HPLC assay method at 0, 24, 48 and 72 hours under above storage condition. As a result, niacinamide, pyridoxine and riboflavin were stable in IVH-1 and IVH-3 solutions within 72 hours. But thiamine was found to be unstable. Percentages of the remaining thiamine after 0, 24, 48 and 72 hours of storage were 89, 83, 79 and 73 respectively in IVH-1 solution. The percentages of the remaining thiamine after 0, 24, 48 hours were 73, 28, 24 respectively in IVH-3 solution, but after 72 hours, the amount of thiamine remaining in IVH-3 solution is below detection range. Therefore a patient received nutritional support by TPN solution, it is recommended that the TPN solution should be used immediately after preparation.
|